Cumate-Inducible Gene Expression System for Sphingomonads and Other Alphaproteobacteria
نویسندگان
چکیده
منابع مشابه
Cumate - inducible gene expression system for sphingomonads 1 and other Alphaproteobacteria 2 3
11 Tunable promoters represent a pivotal genetic tool for a wide range of applications. 12 Here we present such a system for sphingomonads, a phylogenetically diverse group of 13 bacteria that have gained much interest for their potential in bioremediation and their 14 use in industry and for which no dedicated inducible gene expression system has been 15 described so far. A strong, constitutiv...
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OBJECTIVE Transgenic technology is widely used for industrial applications and basic research. Systems that allow for genetic modification play a crucial role in biotechnology for a number of purposes, including the functional analysis of specific genes and the production of exogenous proteins. In this study, we examined and verified the cumate-inducible transgene expression system in chicken D...
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DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and...
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BACKGROUND A number of expression systems have been developed where transgene expression can be regulated. They all have specific characteristics making them more suitable for certain applications than for others. Since some applications require the regulation of several genes, there is a need for a variety of independent yet compatible systems. RESULTS We have used the regulatory mechanisms ...
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Research on the human pathogen Mycobacterium tuberculosis (Mtb) would benefit from novel tools for regulated gene expression. Here we describe the characterization and application of a synthetic riboswitch-based system, which comprises a mycobacterial promoter for transcriptional control and a riboswitch for translational control. The system was used to induce and repress heterologous protein o...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2013
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.02296-13